The U S Department Of Health And Human Services Hhs National Institutes Of Health Nih National Cancer Institute Nci Center For Cancer Research Ccr Experimental Immunology Branch Eib Requires The Procurement Of A Microelectrode Current And Voltage Clamp Amplifier And Digitizer System This Is A Combined Synopsis Solicitation For Commercial Items Prepared In Accordance With The Format In Far 12 6 As Supplemented With Additional Information Included In This Notice And Shall Be Processed In Accordance With Far Part 13 Simplified Acquisition Procedures This Announcement Constitutes The Only Solicitation And A Separate Written Solicitation Will Not Be Issued This Solicitation 75N91021q00032 Is Issued As A Request For Quote Rfq And Includes All Applicable Provisions And Clauses In Effect Through Far Federal Acquisition Circular Fac 2021 03 02 16 2021 Simplified Acquisition Procedures For Commercial Items The North American Industry Classification System Code Is 334516 And The Business Size Standard Is 1 000 Employees This Is A Total Small Business Set Aside 1 0 Brand Name Or Equal This Requirement Is For The Procurement Of The Brand Name Or Equivalent Instruments Described In Section 4 0 The Federal Acquisition Regulation Far Provision Far 52 211 6 Brand Name Or Equal Aug 1999 Is Applicable To This Requirement 2 0 Background The National Cancer Institute Nci Center For Cancer Research Ccr Experimental Immunology Branch Eib Has Established An Electrophysiology Platform To Examine Enteric Neurons And Intestinal Enteroendocrine Cells Eib Has Been Performing Patch Clamp Recordings In A Collaborator S Laboratory Outside Of Eib For The Past Two Years And Have Recently Published A Manuscript About Intestinal Enterochromaffin Cells Immunity In Press Based On These Works Eib Will Continue To Do Electrophysiology Experiments To Study Intestinal Physiology And Pathophysiology Therefore To Continue With These Experiments It Will Be Significantly More Efficient And Appropriate For Eib To Set Up A Rig System In A Eib Laboratory The Rig System Inclusive Of The Required Instruments And Equipment Rack Described In Section 4 0 Is Based On Working Experiences And Specific Laboratory Needs Eib Has Experienced Scientists In Electrophysiology Well Trained In Neuroscience And Are Very Familiar With The Required Instruments Additionally Eib Has Trained Staff Scientists To Operate And Maintain The Rig System Based On The Past Two Years Experience Considering The Substantial Amount Patch Clamp Recordings Eib Are About To Do The Required Equipment Will Improve Our Working Efficiency Significantly 3 0 Type Of Order This Is A Firm Fixed Price Purchase Order 4 0 Required Products Features Salient Characteristics 4 1 The Following Product Features Characteristics Are Required For One 1 Multiclamp 700B Current And Patch Clamp Amplifier Or Equivalent The Instrument Shall Be Able To Use Either An External Trigger Command Or User Programmable Voltage Threshold For Rapid Automatic Mode Switching Between Current And Voltage Clamp For Example By Pre Setting A Voltage Threshold In Current Clamp Mode The Amplifier Shall Automatically Switch To Voltage Clamp Mode When The Membrane Potential Reaches The Threshold Voltage The Instrument Shall Allow For A User Specified Delay To Be Programmed Allowing Further Customization Of Recording Procedures Sudden Changes In Membrane Or Pipette Parameters May Result In Undesirable Oscillations During Whole Cell Recordings To Overcome This The Required Instrument Shall Detect Current Or Voltage Oscillations And Automatically Disables Or Intelligently Reduces Compensation Settings To Protect The Cell From Damage Slight Voltage Drift Often Due To Changing Electrode Properties May Contaminate An Otherwise Decent Current Clamp Recording To
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