Tenders are invited for Supply of Laboratory Consumables Non Refundable Fee 12 500 taxes Closing Date 2020 09 07 FITC Anti Rabies Monoclonal Globulin 5ml vialPacking 1 vialFluorescent Mounting medium Anti fading Packing 1 mlAnti neutrophil cytoplasmic antibody ANCA immunofluorescence kitPacking 1 kitNitro blue tetrazoliumPacking 1 mgOCT compound embedding medium for frozen tissueembeddingPacking 1 mlHuman Genomic DNA Extraction kit 50 reactions kit Packing 1 kitTotal Human RNA Extraction Kit 250reactions kit Packing 1 kitDNA extraction kit to extract DNA for real time PCRtesting from swabs urinePacking 1 TestTotal Human RNA Extraction Kit 50 reactions Packing 1 kitPCR reagent kit to detect Chlamydia trachomatis Neisseria gonorrheaPacking 1 TestPCR reagent kit to detect Herpes simplex virusPacking 1 TestDNA Extraction kit 250 purifications kit 1 The kit should be designed for rapid purification of total DNA including parasite DNA e g genomic mitochondrial and pathogen from Varity of sample sources including fresh or frozen animal tissues and cells blood or bacteria and mosquito samples 2 Kit should be supplied with followings spin columns collection tubes buffers and proteinase K 3 Elution volume should be 100 200 mic l 4 Number of purification per kit should be 250 5 Purification procedure should be based on spin column 6 Maximun sample amount 100 mic l 25mg 7 Technology Silica technology8 Time per run or per preparation 20 minutes 1 hour 9 Should yeald pure DNA DNA Yield 6 mic g PCR Master Mix 5ml Bottle1 Detection method Primer Probe detection 2 Should be compatible with 7300 ABI PCR System 3 Polymerase AmpliTaq Fast DNA polymerase 4 Master mix contains Amplitaq Fast DNA polymerase Uracil N Glycosylase UNG dNTPs with d UTP ROX TM dye passive reference and optimized buffer Packing 1 Bottle30mic g10 The purified DNA should be free of protein nucleases and other contaminants or inhibitors 11 Kit should be supplied with Optimized protocols forpurification of high quality DNA for PCR real timePCR and genotyping Packing 1 kit
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