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Tenders are invited for Reagents, Consumables and Equipment for Molecular Genetic Research Using Nanopore Sequencing. 1.1. Microbial Community DNA Extraction Kit with Mechanical Lysis 2 kits of 50 preparations each Contents: bead-beating tubes, silica-gel columns, lysis, wash and elution buffers, and a reagent or columns for PCR inhibitor removal. Suitable for swabs, tissues, membrane filters and cell pellets. Extraction of DNA from Gram-negative and Gram-positive bacteria and fungi from a single sample. Manufacturer-declared suitability for 16S rRNA and whole-genome shotgun metagenomic sequencing. Reference: ZymoBIOMICS DNA Miniprep Kit with lysis tubes, Zymo Research, Cat. No. D4300. 1.2. DNA Extraction Kit for Soil and Sediments 1 kit, 50 preparations Mechanical lysis included in the kit; removal of humic acids and other PCR inhibitors; column purification. Suitable for soil, sediments and turbid-water concentrates. Reference: Exgene Soil DNA Mini, GeneAll. 1.3. DNA Extraction Kit for Clinical Samples 1 kit, 100 preparations Silica-gel column; Proteinase K and lysis buffer included; suitable for swabs, tissues and biological fluids. Reference: Exgene Clinic SV Mini, GeneAll. 1.4. Total Nucleic Acid Extraction Kit (DNA + RNA) 1 kit, 50 preparations Simultaneous extraction of DNA and RNA from one sample; carrier RNA or equivalent yieldenhancing agent included; completely RNase-free. Reference: Ribospin Pathogen TNA, GeneAll. 1.5. Whole-Cell Microbial Community Standard 1 kit, 10 preparations Mock community consisting of whole cells (not purified DNA), containing at least 8 bacterial and 2 fungal strains, including difficult-to-lyse Gram-positive bacteria and yeasts. The theoretical composition based on genomic DNA must be documented. Packaging: 75 µl per preparation, supplied in a stabilizing solution. Manufacturer-declared suitability for 16S and shotgun metagenomics. Reference: ZymoBIOMICS Microbial Community Standard, Zymo Research, Cat. No. D6300. 1.6. Microbial Community Standard as Purified Genomic DNA 1 vial, at least 200 ng A mixture of genomic DNA from the same eight bacterial and two fungal strains as in Item 1.5, with an identical theoretical composition. Concentration at least 10 ng/µl; foreign microbial DNA not more than 0.01%; genomic GC-content range 1585%; documented composition with lot number. Reference: ZymoBIOMICS Microbial Community DNA Standard, Zymo Research, Cat. No. D6305 (200 ng) or D6306 (2,000 ng). 2. Reagents and Consumables for Library Preparation 2.1. Flow Cell Wash Kit 1 kit Oxford Nanopore Technologies, Cat. No. EXP-WSH004. Equivalent not allowed: the reagent is proprietary, compatible exclusively with Oxford Nanopore Technologies flow cells, and the manufacturer is the sole source of supply. 2.2. Tubes for Qubit Fluorometer 500 pcs 0.5 ml thin-wall transparent tubes, optically compatible with Qubit 4 fluorometer; DNase- and RNase-free. Reference: Invitrogen, Cat. No. Q32856. 2.3. Bovine Serum Albumin (BSA), 50 mg/ml 1 vial, at least 1 ml Ultrapure, molecular-grade, nuclease-free and suitable for PCR. Reference: Invitrogen UltraPure BSA, Cat. No. AM2616. 2.4. Paramagnetic Beads for Amplicon and Library Purification 5 ml Size-selective binding of double-stranded DNA; removal of unused nucleotides, primers, primer dimers and salts; salt-free elution; effective recovery of amplicons over 100 bp; confirmed compatibility with Oxford Nanopore Technologies library preparation protocols; shelf life at least 12 months. Reference: AMPure XP, Beckman Coulter, Cat. No. A63880. 2.5. Absolute Ethanol, Molecular Grade 1 litre Concentration at least 99.5%, DNase- and RNase-free, for preparation of fresh 80% solution. 2.6. Low-DNA-Binding Tubes and PCR Tubes 200 pcs of each size 1.5 ml tubes 200 pcs 2.0 ml tubes 200 pcs 0.2 ml thin-wall PCR tubes, individual or strips 200 pcs Requirements: manufacturer-certified reduced DNA adsorption (LoBind or equivalent) for 1.5 and 2.0 ml tubes; DNase-, RNase-, human-DNA- and PCR-inhibitor-free; centrifugation capability of at least 20,000 × g for 1.5 and 2.0 ml tubes; secure caps that do not open during heating. Reference: Eppendorf DNA LoBind Tubes. 2.7. 96-Well Low-DNA-Binding Plates and Sealing Films 10 pcs each 0.2 ml plates compatible with the thermocycler in Item 3.1 and magnetic rack in Item 3.6; heatsealing or adhesive films suitable for thermal cycling, DNase- and RNase-free. 3. Equipment 3.1. Thermocycler 2 pcs Heated lid with adjustable temperature; block for 16 × 0.2 ml tubes or 96-well plate; operating temperature range 499°C; temperature accuracy no worse than ±0.5°C; temperature ramp rate at least 2°C/s; programming of multi-step protocols with memory storage; 220240 V, 50 Hz; compact design suitable for a mobile laboratory. Reference: miniPCR mini16x. 3.2. Microcentrifuge 1 pc Rotor for 12 × 1.5/2.0 ml tubes; maximum RCF at least 16,000 × g; timer; lid lock during rotation; 220240 V power supply. Reference: Biosan Microspin 12 Plus, Cat. No. BS-010218-AA1. 3.3. Centrifuge for PCR Strips and Plates 1 pc Interchangeable rotors for 0.2 ml strips and 96-well plates; RCF at least 500 × g; automatic start when the lid is closed. 3.4. Homogenizer (Bead Beater) 1 pc with adapters Compatible with 2.0 ml lysis tubes containing beads (ZR BashingBead type); simultaneous processing of at least 12 tubes; adjustable time and intensity; adapter or holder included. Reference: Homogenizer compatible with the kit in Item 1.1. 3.5. Vortex Mixer 2 pcs with adapters Speed at least 2,500 rpm; continuous and touch-operation modes; adjustable speed; adapter for 1.5/2.0 ml tubes included. Reference: Sigma-Aldrich, Cat. No. Z653438. 3.6. Magnetic Rack for PCR Strips and 96-Well Plates 1 pc 0.2 ml format; neodymium magnets; housing resistant to disinfectants and alcohols. Racks for 1.5/2.0 ml tubes are already available to the purchaser and are not being procured. 3.7. Mechanical Pipettes 14 pcs Single-channel variable-volume: 0.12 µl 2 pcs 0.510 µl 4 pcs 10100 µl 4 pcs Eight-channel variable-volume: 220 µl 2 pcs 20200 µl 2 pcs Requirements: autoclavable as a complete unit or lower section; user calibration capability; accuracy and repeatability compliant with ISO 8655; compatible with universal filter tips; service and calibration support available in Ukraine. Pipettes of 220, 20200 and 1001,000 µl are already available to the purchaser and are not being procured. 3.8. Filter Tips 6,720 pcs Nominal volume 10 µl, extended with fine tip, for 0.12 and 0.510 µl pipettes 1,920 pcs; 100 µl 1,920 pcs; 20 µl 960 pcs; 200 µl 960 pcs; 1,000 µl 960 pcs. Requirements: sterile, hydrophobic filter, low adsorption, free from DNases, RNases, human DNA and PCR inhibitors, supplied in racks. Compatibility with the pipettes in Item 3.7, including the 0.12 µl pipette, must be confirmed by the pipette manufacturer. If necessary, special micro-volume tips recommended by the manufacturer for this range shall be supplied. 3.9. Water Filtration Equipment 1 set (preferably 2 lines) Holder or manifold for 47 mm membrane filters, autoclavable, or sterile disposable filtration units; vacuum pump with trap flask or syringe filtration system; sterile forceps for membrane transfer at least 4 pcs. 3.10. UV Decontamination Cabinet (PCR Cabinet) 1 pc Working chamber for pre-PCR preparation with 254 nm germicidal lamp and automatic shut-off timer. Dimensional limitation: must fit on a work surface measuring 53 × 170 cm; therefore cabinet width must not exceed 160 cm and depth must not exceed 50 cm. Disassemblable construction, weight no more than 5 kg, capable of being stored disassembled. Materials resistant to alcohols, hypochlorite and commercial DNA decontaminants. Acceptable designs: disassemblable cabinet with UV lamp, flexible film cabinet or dome cabinet. 4. Consumables for Sample Collection, Concentration and Preservation 4.1. Sterile Flocked Swabs 200 pcs Synthetic flocked swabs in individual sterile packaging, with plastic shaft and breakpoint; without transport medium containing PCR inhibitors. Suitability for molecular testing must be declared by the manufacturer. 4.2. Sterile 0.9% Sodium Chloride Solution 100 units of 510 ml each Single-use ampoules or vials, sterile and preservative-free. 4.3. Sterile 2.0 ml Screw-Cap Tubes 200 pcs With sealing ring, suitable for transportation of swabs and membrane filters, DNase- and RNase-free, with labelling area. 4.4. Sterile 1-Litre Water Sampling Bottles 40 pcs Polymer or glass, screw cap, sterile, disposable or autoclavable. At least 20 pcs must contain sodium thiosulfate to neutralize residual disinfectant in chlorinated water. 4.5. 47 mm Membrane Filters 100 pcs of Each Pore Size 0.22 µm 100 pcs 0.45 µm 100 pcs Sterile, individually packaged, made of mixed cellulose esters or polyethersulfone, compatible with the holder in Item 3.9 and the lysis buffers of the kits in Items 1.11.3. 4.6. Nucleic Acid Stabilization Solution at least 100 ml concentrate or 100 ready-to-use 1 ml tubes Inactivates nucleases and preserves DNA and RNA at ambient temperature for at least 30 days; compatible with column-based extraction kits. Reference: DNA/RNA Shield, Zymo Research. 4.7. Thermal Container with Cold Packs 2 sets Thermal container with a capacity of at least 10 litres, with cold packs capable of maintaining 28°C for at least 12 hours. [Disclaimer: The above text is machine translated. For accurate information kindly refer the original document.] Tender Link : http://aph.org.ua/en/tenders/programme-tenders/
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