Contract Notice Laboratory Optical And Precision Equipments Excl Glasses Delivery Of A Flow CytometerDescription Specificationsspectral Flow Cytometer For Comprehensive Phenotypic And Functional Analysis Of Cells Bacteria And Particles In Suspension With The Help Of The Spectral Flow Cytometer Primarily Infected And Non Infected Immune Cells Are To Be Characterized Among Other Things T Lymphocytes Are To Be Profoundly Profound In Humans And Animals For This It Is Necessary To Use 30 Or More Different Monoclonal Antibodies Simultaneously These Include For Example Antibodies To The Following Molecules Markers T Cell Subpopulations Cd3 Cd4 Cd8 Tcr Alpha Tcr Alpha Tcr Alpha Cd56 Klrg1 Naive Vs Memory Vs Effector Cells Cd62l Cd44 Cd45ra Cd45ro Cd27 Cd28 Cd69 Costimulatory Molecules Ctla4 Icos Fas Pd 1 Cd25 Cd127 Chemokine Receptors Ccr7 Ccr4 Cxcr3 Cxcr4 Cxcr5 Ccr6 Cx3cr1 Apoptosis Living Dead Differentiation Annexin Live Dye Z B Dapi Power Requirement Number Of Fluorochromes To Be Detected Simultaneouslyability To Simultaneously Detect At Least 30 Different Fluorochromes Colors That Can Be Conjugated To Antibodies Possibility Of Subtraction Of Autofluorescence Possibility To Efficiently Evaluate New Fluorochromes And A High Degree Of Flexibility For Easy Evaluation Of Different Fluorochrome Combinations No Need For Complex Compensation Matrices With Simultaneous Use Of 30 Colors Specific Features And Design The Device Is Equipped With The Required Number Of Lasers In The Range Of Uv Violet Blue Yellow Green And Red To Achieve Said Resolution Sensitivity So That It Can Analyze 30 Colors With Commercially Available Fluorochromes Spatial Separation Of The Different Laser Detection Modules High Measuring Stability Over Time Information About Instrument Stability Over Time Including Laser Delay Voltage Or Gain Variations Should Be Readily Available To The User The Cell Carryover Should Beavailability Of A Plate Reader Preferably For 96 Wells And 384 Wells Possibility Of Volumetric Control To Allow Absolute Quantification Of Samples Without Beads Acquisition Speed And Abort Rate To Be Reached When Measuring Cells Ability To Acquire At Least 25 000 Cells Second At A Kill Rate Of 10 Preferably Less In The Acquisition Of At Least 32 Parameters 30 Fluorescence Parameters And 2 Scatter Parameters With Consistent Sample Quality I E No Significant Loss Of Cell Population Separation Ability To Acquire At Least 35 000 Cells Second At A Kill Rate Of 15 Preferably Less In The Acquisition Of At Least 32 Parameters 30 Fluorescence Parameters And 2 Scattering Parameters With Consistent Sample Quality I E No Significant Loss Of Cell Population Separationelectronics And Software Simple Instrument Setup Procedures Including Calibration And Compensation For A Variety Of Multi Color Experiments So For Example The Acquisition Software Includes Automatic Compensation Demixing And Allows The Use Of Previously Purchased Reference Controls The Software Should Be Compatible With Windows 10 Efficient Capture And Processing Of Files With 10 Million Cells Per Sample In Single Files For Further Analysisthe Output Of The Files Should Be Available In Fsc 3 0 Or Fcs 3 1 File For Further Analysis And Be Compatible With Widely Used Analysis Software Programs E G Flowjo Infinicyt And Fcs Express Ability To Record Information About The Hardware Such As Laser Functions In The Raw Data File Training Hardware And Software Should Be User Friendly And Intuitive To Use Time Limit For Receipt Of Tenders Or Requests To Participate Date 23 09 2019 Local Time 12 00 Disclaimer The Above Text Is Machine Translated For Accurate Information Kindly Refer The Original Document
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